2HLV

Bovine Odorant Binding Protein deswapped triple mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 41 21 2
Unit cell lengths	83.501, 83.501, 48.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.650
R-factor	0.18589
Rwork	0.185
R-free	0.21224
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g85
RMSD bond length	0.012
RMSD bond angle	1.317
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.000	1.700
High resolution limit [Å]	1.650	1.650
Number of reflections	21290
<I/σ(I)>	5.2	2
Completeness [%]	99.9	99.8
Redundancy		20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	26% (W/V) PEG 8000, 200 mM Ammonium Sulfate and 10 mM Na Acetate (pH 4.8), protein at 7.5 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 298K