2HLF
Structure of the Escherichis coli ClC chloride channel Y445E mutant and Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91921 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.835, 97.593, 171.541 |
| Unit cell angles | 90.00, 131.52, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.300 |
| R-factor | 0.28225 |
| Rwork | 0.281 |
| R-free | 0.30879 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ots |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 42596 | |
| Completeness [%] | 95.8 | 81.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 34% peg 200/300 1:2, 50mM glycin, 150mM NaBr, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
