2HKP
SUMO protease Ulp1 with the catalytic cysteine oxidized to a sulfenic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.089, 40.328, 55.379 |
| Unit cell angles | 90.00, 112.02, 90.00 |
Refinement procedure
| Resolution | 51.367 - 2.100 |
| Rwork | 0.216 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1euv |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.970 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.367 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.493 |
| Total number of observations | 6418 | |
| Number of reflections | 14736 | |
| <I/σ(I)> | 5.9 | 1.5 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 20% w/v PEG 3350, 0.2M NaCl and post-soaked in 50mM DTT for 48 hours, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
