2HKE
Mevalonate diphosphate decarboxylase from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.463, 168.666, 54.850 |
| Unit cell angles | 90.00, 118.78, 90.00 |
Refinement procedure
| Resolution | 42.180 - 1.800 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fi4 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.558 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.180 | 42.170 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
| Rmerge | 0.106 | 0.092 | 0.658 |
| Total number of observations | 13689 | 62870 | |
| Number of reflections | 74805 | ||
| <I/σ(I)> | 4.3 | 6.5 | 1.1 |
| Completeness [%] | 99.0 | 98.6 | 98 |
| Redundancy | 6.1 | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 22% PEG 8000, 180mM ammonium sulfate, 100mM sodium cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
