2HJR
Crystal Structure of Cryptosporidium parvum malate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-25 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 32 |
| Unit cell lengths | 171.887, 171.887, 135.606 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.830 - 2.200 |
| R-factor | 0.20048 |
| Rwork | 0.198 |
| R-free | 0.24747 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1guy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.117 | 0.864 |
| Number of reflections | 227351 | |
| <I/σ(I)> | 14.6 | 1.911 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 15% PEG4K, 0.2M NH4oAC, Nacitrate pH 5.6, 15% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
