2HJO

Crystal structure of V224H design intermediate for GFP metal ion reporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-12-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9188
Spacegroup name	P 21 21 21
Unit cell lengths	51.630, 62.150, 69.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.250
R-factor	0.128
Rwork	0.128
R-free	0.16500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
RMSD bond length	0.012
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.290
High resolution limit [Å]	1.250	1.250
Number of reflections	59175
<I/σ(I)>	27.1	4.6
Completeness [%]	95.5	72.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	19% PEG 4K, 50 mM MgCl2, 50 mM Hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K