2HJN
Structural and functional analysis of Saccharomyces cerevisiae Mob1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795, 0.9792, 0.9600 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.750, 70.811, 50.622 |
| Unit cell angles | 90.00, 119.35, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.000 |
| R-factor | 0.20421 |
| Rwork | 0.202 |
| R-free | 0.23747 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.760 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.050 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.278 |
| Number of reflections | 14415 | |
| <I/σ(I)> | 18.4 | 2.9 |
| Completeness [%] | 99.5 | 95 |
| Redundancy | 5.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 1.2M ammonium sulfate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
