2HJG
The crystal structure of the B. subtilis YphC GTPase in complex with GDP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9794, 0.9797, 1.0094 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.711, 65.045, 110.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.500 |
| R-factor | 0.21815 |
| Rwork | 0.215 |
| R-free | 0.27353 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.251 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.098 | 0.320 |
| Number of reflections | 16253 | |
| <I/σ(I)> | 17.8 | 5.1 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 0.2M sodium flouride, 20% (w/v) PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
