2HIM
Crystal Structure and Allosteric Regulation of the Cytoplasmic Escherichia coli L-Asparaginase I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.524, 89.766, 93.290 |
| Unit cell angles | 90.00, 117.14, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.820 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 132353 | |
| <I/σ(I)> | 14.8 | 1.5 |
| Completeness [%] | 98.3 | 86 |
| Redundancy | 3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | Citrate buffer, Sodium Chloride, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
