2HHH
Crystal structure of kasugamycin bound to the 30S ribosomal subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 410.440, 410.440, 172.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.740 - 3.350 |
| R-factor | 0.268 |
| Rwork | 0.265 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EMPTY 30S |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.400 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 205944 | |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 98.4 | 91.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | MPD, MAGNESIUM CHLORIDE, POTASSIUM CHLORIDE, AMMONIUM CHLORIDE, MES-KOH, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | AMMONIUM CHLORIDE | ||
| 11 | 1 | 2 | MES-KOH | ||
| 12 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | MAGNESIUM CHLORIDE | ||
| 3 | 1 | 1 | POTASSIUM CHLORIDE | ||
| 4 | 1 | 1 | AMMONIUM CHLORIDE | ||
| 5 | 1 | 1 | MES-KOH | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 2 | MAGNESIUM CHLORIDE | ||
| 9 | 1 | 2 | POTASSIUM CHLORIDE |
