2HH9
Thiamin pyrophosphokinase from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.309, 60.696, 64.831 |
| Unit cell angles | 65.94, 89.86, 64.87 |
Refinement procedure
| Resolution | 50.190 - 2.100 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g9z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.740 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.047 | 0.083 |
| Number of reflections | 35489 | |
| <I/σ(I)> | 18.5 | 8.9 |
| Completeness [%] | 96.1 | 85.4 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG4000, 0.2M Magnesium chloride, 0.1M Tris, 20% Glycerol. Protein incubated with Thiman, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
