2HH0

Structure of an Anti-PrP Fab, P-Clone, in Complex with its Cognate Bovine Peptide Epitope.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 31 2 1
Unit cell lengths	119.834, 119.834, 95.534
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.391 - 2.850
R-factor	0.222
Rwork	0.222
R-free	0.28200
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.410
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.391	43.390	3.000
High resolution limit [Å]	2.850	9.010	2.850
Rmerge	0.068	0.056	0.298
Total number of observations		1493	7316
Number of reflections	18459
<I/σ(I)>	7.7	9.6	2.6
Completeness [%]	98.4	92.1	100
Redundancy	2.7	2.5	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	Well: 57% saturated ammonium sulfate Drop: 10/1 protein solution/well solution, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K