2HG4
Structure of the ketosynthase-acyltransferase didomain of module 5 from DEBS.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9392, 0.9793, 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 305.258, 150.149, 184.378 |
| Unit cell angles | 90.00, 110.03, 90.00 |
Refinement procedure
| Resolution | 48.030 - 2.730 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.458 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.170 | 2.880 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.112 | 0.680 |
| Number of reflections | 206652 | |
| <I/σ(I)> | 8.3 | 1.9 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG4000, 0.2M Lithium Sulfate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
