2HFU
Crystal structure of L. major mevalonate kinase in complex with R-mevalonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-29 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.185, 88.446, 87.718 |
| Unit cell angles | 90.00, 103.51, 90.00 |
Refinement procedure
| Resolution | 36.490 - 2.000 |
| R-factor | 0.17905 |
| Rwork | 0.176 |
| R-free | 0.23016 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Selenomethionine derivative of the L. major mevalonate kinase |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.952 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.253 | 2.110 |
| High resolution limit [Å] | 1.700 | 2.000 |
| Rmerge | 0.070 | 0.297 |
| Number of reflections | 39420 | |
| <I/σ(I)> | 9 | 2.5 |
| Completeness [%] | 95.6 | 89.4 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 1.2M sodium citrate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
