2HF0
Bifidobacterium longum bile salt hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-30 |
| Wavelength(s) | 1.5148 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 123.981, 123.981, 219.565 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.18699 |
| Rwork | 0.185 |
| R-free | 0.22835 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.100 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.211 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 46662 | |
| Completeness [%] | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 303 | ammonium sulfate 30% w/v, 0.5 M sodium formate, sucrose (1M) 100ml, 40 uL of b-octyl glucosidase (0.50% w/v), pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
