2HDI
Crystal structure of the Colicin I receptor Cir from E.coli in complex with receptor binding domain of Colicin Ia.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.633, 130.494, 56.261 |
Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.500 |
R-factor | 0.19097 |
Rwork | 0.188 |
R-free | 0.24929 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hdf |
RMSD bond length | 0.010 |
RMSD bond angle | 1.303 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.103 | 0.570 |
Number of reflections | 32311 | |
<I/σ(I)> | 15.3 | 2.73 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 294 | 9 G/L PROTEIN IN 0.02M TRIS PH7.5, 0.2M NACL, 0.05% LDAO, 0.45% C8E4, 3% HEPTANETRIOL MIXED AT 1:1 RATIO WITH 0.1M MES PH6.2, 10% GLYCEROL, 22% PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 294K |