2HD5
USP2 in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-15 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.903, 45.534, 76.360 |
| Unit cell angles | 90.00, 110.01, 90.00 |
Refinement procedure
| Resolution | 41.470 - 1.850 |
| Rwork | 0.177 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1OGW and 1NBF chain C |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.200 | 1.930 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 28474 | |
| <I/σ(I)> | 16.1 | 4 |
| Completeness [%] | 95.5 | 81.8 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 295 | 200 mM MgCl2, 25% PEG 3550, 100 mM Bis-Tris, Protein at 11.8 mg/ml, pH 6.5, LIQUID DIFFUSION, temperature 295K |
