2HCN
Crystal structure of RNA dependent RNA polymerase domain from west nile virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 43 |
| Unit cell lengths | 110.064, 110.064, 68.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.820 - 2.350 |
| R-factor | 0.20962 |
| Rwork | 0.207 |
| R-free | 0.25944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WEST NILE VIRUS RNA DEPENDANT RNA POLYMERASE COORDINATES 2HCS (DOMAIN FROM AMINO ACIDS 317-905) |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.729 |
| Data reduction software | MOSFLM |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.000 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 34338 | |
| <I/σ(I)> | 21.8 | 1.5 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% peg 8000, Imid 0.1M, Ca(OAc)2 0.2M, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
