2HCI
Structure of Human Mip-3a Chemokine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-15 |
| Detector | SBC-2 |
| Wavelength(s) | 0.95370 |
| Spacegroup name | I 4 |
| Unit cell lengths | 83.991, 83.991, 57.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.760 - 1.810 |
| R-factor | 0.20944 |
| Rwork | 0.206 |
| R-free | 0.25450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m8a |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.856 |
| Data reduction software | DENZO |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.570 | 1.990 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.062 | 0.308 |
| Number of reflections | 17465 | |
| <I/σ(I)> | 9.1 | 2.4 |
| Completeness [%] | 100.0 | 16.6 |
| Redundancy | 3.47 | 1.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.6M ammonium sulfate, 10% v/v dioxane, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
