2HBK
Structure of the yeast nuclear exosome component, Rrp6p, reveals an interplay between the active site and the HRDC domain; Protein in complex with Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.50 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.680, 110.680, 79.729 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.220 - 2.250 |
| R-factor | 0.228 |
| Rwork | 0.222 |
| R-free | 0.27900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2hbj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.271 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.220 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.040 | 0.341 |
| Number of reflections | 27056 | 2637 |
| <I/σ(I)> | 19.7 | 4.9 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 12-14% PEG 20000, 0.1M Mes or Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
