2HBJ

Structure of the yeast nuclear exosome component, Rrp6p, reveals an interplay between the active site and the HRDC domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-10
Detector	MARRESEARCH
Wavelength(s)	0.8793, 1.072, 1.071, 1.5218
Spacegroup name	P 31 2 1
Unit cell lengths	110.135, 110.135, 80.169
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.060 - 2.100
R-factor	0.239
Rwork	0.232
R-free	0.29200
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.348
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.060	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.058	0.503
Number of reflections	33892	3087
<I/σ(I)>	12.7	2.6
Completeness [%]	98.5	91.3
Redundancy	4.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	12-14% PEG 20000, 0.1M Mes or Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	7	277	12-14% PEG 20000, 0.1M Mes or Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K