2HAY
The Crystal Structure of the Putative NAD(P)H-Flavin Oxidoreductase from Streptococcus pyogenes M1 GAS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.205, 91.874, 175.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.710 - 2.110 |
| R-factor | 0.18034 |
| Rwork | 0.175 |
| R-free | 0.23246 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.434 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.138 | 0.430 |
| Number of reflections | 55236 | |
| <I/σ(I)> | 9.2 | 4.04 |
| Completeness [%] | 96.2 | 73.5 |
| Redundancy | 8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 282 | 0.2M Ammonium Sulfate 0.1M MES pH 6.5 30% PEG 5000 MME and Flavin, VAPOR DIFFUSION, SITTING DROP, temperature 282K |
