2HA9
Crystal structure of protein SP0239 from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.906, 139.963, 148.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.19754 |
| Rwork | 0.194 |
| R-free | 0.27127 |
| Structure solution method | SAD |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.954 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.104 | 0.694 |
| Number of reflections | 33065 | |
| <I/σ(I)> | 23.14 | 1.82 |
| Completeness [%] | 95.5 | 85 |
| Redundancy | 6.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2 M NaCl, 0.1M Bis-Tris, 25 % PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
