2H85
Crystal Structure of Nsp 15 from SARS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-11-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 166.584, 166.584, 166.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.700 - 2.600 |
| R-factor | 0.2092 |
| Rwork | 0.208 |
| R-free | 0.24008 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.301 |
| Data reduction software | MOSFLM |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 2.667 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.169 | 0.778 |
| Number of reflections | 24505 | |
| <I/σ(I)> | 11.8 | 4 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 11.8 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 297 | 5-20% PEG 10000, 0.2M imidazole malate pH 9.0-10.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
