2H7V

Co-crystal structure of YpkA-Rac1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	80
Detector technology	CCD
Collection date	2005-04-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 1
Unit cell lengths	66.398, 75.519, 99.772
Unit cell angles	92.08, 103.38, 115.79

Refinement procedure

Resolution	95.780 - 2.600
R-factor	0.222
Rwork	0.222
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 2H7O 1mh1
RMSD bond length	0.017
RMSD bond angle	1.666
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.780	2.667
High resolution limit [Å]	2.600	2.600
Number of reflections	51987
<I/σ(I)>	11.8	3
Completeness [%]	99.4	99.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	100mM HEPES pH 6.5-7.5, 5%-8% PEGMME2000 micro seeding, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	100mM HEPES pH 6.5-7.5, 5%-8% PEGMME2000 micro seeding, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	100mM HEPES pH 6.5-7.5, 5%-8% PEGMME2000 micro seeding, VAPOR DIFFUSION, SITTING DROP, temperature 295K