2H77
Crystal structure of human TR alpha bound T3 in monoclinic space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.569, 80.626, 62.614 |
| Unit cell angles | 90.00, 121.09, 90.00 |
Refinement procedure
| Resolution | 32.220 - 2.330 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.617 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 62.994 | 35.540 | 2.460 |
| High resolution limit [Å] | 2.330 | 7.370 | 2.330 |
| Rmerge | 0.086 | 0.048 | 0.383 |
| Total number of observations | 1068 | 4767 | |
| Number of reflections | 18468 | ||
| <I/σ(I)> | 7.7 | 10.1 | 2 |
| Completeness [%] | 86.0 | 71.9 | 90 |
| Redundancy | 1.8 | 2.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 1.0 M sodium cacodylate and 0.1M sodium acetate threehydrate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
