2H5R
Crystal structure of mStrawberry at pH 10.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.646, 44.177, 61.179 |
| Unit cell angles | 90.00, 105.98, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.034 |
| Data scaling software | HKL-2000 |
| Phasing software | TNT |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 30663 | |
| Completeness [%] | 100.0 | 64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 298 | 100 mM CAPS pH 11.5, 1.1M NaCitrate, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
