2H5G
Crystal structure of human pyrroline-5-carboxylate synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 122.022, 137.402, 72.057 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.560 - 2.250 |
| R-factor | 0.23248 |
| Rwork | 0.230 |
| R-free | 0.27211 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.478 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.290 | 2.350 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.056 | 0.261 |
| Number of reflections | 57217 | |
| <I/σ(I)> | 4.39 | |
| Completeness [%] | 98.2 | 88.3 |
| Redundancy | 6.89 | 3.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1% PEG 3350, 1.0M (NH4)2SO4, 0.1M BIS-TRIS, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
