2H57

Crystal structure of human ADP-ribosylation factor-like 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-05-16
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	80.058, 134.885, 63.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.20749
Rwork	0.206
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1zd9 2h16
RMSD bond length	0.018
RMSD bond angle	1.393
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.043	0.019	0.599
Number of reflections	46561	4994	4483
<I/σ(I)>	17.6
Completeness [%]	97.9
Redundancy	5.3	5.1	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	2M ammonium sulfate, 0.2M sodium chloride, 0.1M sodium cacodylate, pH 5.5, vapor diffusion, sitting drop, temperature 291K