2H36
Structure of ORF14 from Sulfolobus Islandicus Filamentous Virus (SIFV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-04 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9798 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 68.099, 68.099, 132.433 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.070 - 2.950 |
R-factor | 0.23353 |
Rwork | 0.227 |
R-free | 0.29442 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.428 |
Data reduction software | MOSFLM |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | 3.110 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.070 | 0.386 |
Number of reflections | 4170 | |
<I/σ(I)> | 75 | 17 |
Completeness [%] | 99.3 | 99.4 |
Redundancy | 53.4 | 55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 18-26% (w/v) polyethylene glycol 4000, 0.5M MgCl2, 33mM HEPES, 0.2M imidazole malate pH 5.2-5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 5.3 |