2H2T
CD23 Lectin domain, Calcium 2+-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2004-03-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.02336 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.310, 51.261, 58.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.630 - 1.300 |
| R-factor | 0.16953 |
| Rwork | 0.168 |
| R-free | 0.20045 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h2r |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.658 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 30523 | |
| <I/σ(I)> | 41.6 | 3.5 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 17% (w/v) PEG 4000, 100 mM Mes buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
