2H24
Crystal structure of human IL-10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 36.370, 36.370, 220.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| Rwork | 0.204 |
| R-free | 0.25400 |
| Structure solution method | MIR |
| Starting model (for MR) | 1inr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.314 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.080 |
| Number of reflections | 10087 |
| Completeness [%] | 93.0 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 35% ammonium sulfate, 0.1 M Hepes, pH 7.2 |
