2H1P

THE THREE-DIMENSIONAL STRUCTURES OF A POLYSACCHARIDE BINDING ANTIBODY TO CRYPTOCOCCUS NEOFORMANS AND ITS COMPLEX WITH A PEPTIDE FROM A PHAGE DISPLAY LIBRARY: IMPLICATIONS FOR THE IDENTIFICATION OF PEPTIDE MIMOTOPES

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1995-09
Detector	SIEMENS
Spacegroup name	P 1
Unit cell lengths	43.790, 51.250, 60.180
Unit cell angles	91.82, 98.57, 107.84

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.186 *
Rwork	0.186
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1FLR (FAB 4-4-20)
RMSD bond length	0.017
RMSD bond angle	1.850
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.065
Number of reflections	19345
<I/σ(I)>	15.3
Completeness [%]	90.0	79
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		18% PEG 8K, PH 8.5, 1% GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	18 (%)
2	1	reservoir	Tris	0.1 (M)
3	1	reservoir	glycerol	1 (%)