2H1O
Structure of FitAB bound to IR36 DNA fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.040, 82.403, 135.503 |
| Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
| Resolution | 70.360 - 3.000 |
| R-factor | 0.269 |
| Rwork | 0.212 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h1c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.360 | 3.140 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Number of reflections | 33243 | |
| <I/σ(I)> | 3.1 | 44 |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1 M sodium acetate, pH 4.0, 0.27 M sodium acetate, pH 7.0, 7.2 % PEG 20,000, 7.2 % PEG monomethyl ether 550, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium acetate | ||
| 2 | 1 | 1 | PEG | ||
| 3 | 1 | 1 | PEG monomethyl ether | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | sodium acetate | ||
| 6 | 1 | 2 | PEG | ||
| 7 | 1 | 2 | PEG monomethyl ether |
