2H1M
Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978948 |
| Spacegroup name | H 3 |
| Unit cell lengths | 46.314, 46.314, 128.348 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.21352 |
| Rwork | 0.212 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Similar RNA structure from the crystal grown in different conditions. |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.125 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 2227 | |
| <I/σ(I)> | 12.7 | 6.5 |
| Completeness [%] | 97.2 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 1.0 M Li2SO4, 0.1 M Na-citrate pH 5.6, 0.5 M ((NH4))2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Li2SO4 | ||
| 2 | 1 | 1 | Na-citrate | ||
| 3 | 1 | 1 | (NH4))2SO4 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Li2SO4 | ||
| 6 | 1 | 2 | Na-citrate | ||
| 7 | 1 | 2 | H2O |
