2H13
Crystal structure of WDR5/histone H3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-11-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97750 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.680, 98.860, 80.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.150 - 1.580 |
| R-factor | 0.16769 |
| Rwork | 0.166 |
| R-free | 0.19973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.684 |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.750 | 1.630 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 42966 | |
| Completeness [%] | 98.8 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20-30% PEG3350, 0.1 M HEPES, pH 7.5, 0.05 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP |
