2H0K
Crystal Structure of a Mutant of Rat Annexin A5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-21 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.108, 67.184, 112.313 |
| Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
| Resolution | 33.590 - 2.760 |
| R-factor | 0.201 |
| Rwork | 0.197 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a8a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.590 | 2.910 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Number of reflections | 19089 | |
| <I/σ(I)> | 5.3 | 2 |
| Completeness [%] | 97.1 | 88.8 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | PEG 8000, CALCIUM ACETATE, SODIUM CACODYLATE, SODIUM AZIDE, PH 6.4, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293.0K, pH 6.40 |
