2H0A
Crystal structure of probable Transcription regulator from Thermus thermophilus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00, 0.9789, 0.9793, 0.9950 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 74.450, 74.450, 138.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.790 - 2.800 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.086 | 0.539 |
Number of reflections | 22920 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.2 | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Ethanol, Tris, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |