2GZW
Crystal structure of the E.coli CRP-cAMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2006-03-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.370, 57.150, 90.740 |
| Unit cell angles | 91.25, 101.95, 103.70 |
Refinement procedure
| Resolution | 19.930 - 2.210 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i5z |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.097 | 0.246 |
| Number of reflections | 39942 | |
| Completeness [%] | 92.1 | 96.6 |
| Redundancy | 1.6 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | MG ACETATE, AMMONIUM ACETATE, PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
