2GZM
Crystal Structure of the Glutamate Racemase from Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.346, 90.518, 116.799 |
| Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.990 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1B74 fragments |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.422 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.125 | 0.557 |
| Number of reflections | 77916 | |
| <I/σ(I)> | 7.47 | 2.1 |
| Completeness [%] | 94.5 | 91.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | Protein conc of 8 mG/mL, 50mM sodium glutamate, 2mM DTT, 5% PEG6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
