2GZD
Crystal Structure of Rab11 in Complex with Rab11-FIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97865 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.364, 91.149, 113.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.440 |
| R-factor | 0.22886 |
| Rwork | 0.226 |
| R-free | 0.27888 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rab11 in complex with Rab11-FIP in the trigonal space group |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.974 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.102 | 0.421 |
| Number of reflections | 24064 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 13 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | Ammonium phosphate buffer, between 0.1 and 0.5 M concentration, VAPOR DIFFUSION, temperature 298K |
