2GZ5
Human Type 1 methionine aminopeptidase in complex with ovalicin at 1.1 Ang
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.568, 77.689, 48.644 |
| Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.100 |
| R-factor | 0.135 |
| Rwork | 0.117 |
| R-free | 0.14500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3k |
| RMSD bond length | 0.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | GLRF |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.050 | 0.311 |
| Number of reflections | 244734 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 95.5 | 65.2 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 4-6% PEG 10,000, 100 mM HEPES, pH 6.0 in equal volume of the protein 10 mg/ml in 25 mM HEPES, pH 8.0, 5 mM methionine and 150 mM KCl. To the apo crystals, 1mM cobalt chloride and 2 mM ovalicin were added, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
