2GXA

Crystal structure of papillomavirus E1 hexameric helicase with ssDNA and MgADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 1
Unit cell lengths	100.510, 100.881, 125.023
Unit cell angles	92.60, 111.46, 106.01

Refinement procedure

Resolution	44.650 - 3.150
R-factor	0.239
Rwork	0.239
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	composite of PDB ENTRY 1TUE and unpublished structure
RMSD bond length	0.009
RMSD bond angle	1.400
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.150	3.150
Rmerge	0.100	0.718
Number of reflections	74589
<I/σ(I)>	10.5	1.2
Completeness [%]	98.6	98.1
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	100 mM KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	KCl
2	1	1	H2O
3	1	2	KCL
4	1	2	H2O