2GX8
The Crystal Structure of Bacillus cereus protein related to NIF3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.94642 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.036, 95.036, 260.732 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.960 - 2.200 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.26123 |
Structure solution method | SIRAS |
RMSD bond length | 0.014 |
RMSD bond angle | 1.700 |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.030 | 0.325 |
Number of reflections | 68204 | |
<I/σ(I)> | 34.89 | 3.69 |
Completeness [%] | 97.0 | 86.7 |
Redundancy | 5.48 | 3.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M HEPES, 0.25M NaCl, 25%w/v PEG1000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |