2GX2
Crystal structural and functional analysis of GFP-like fluorescent protein Dronpa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 73.091, 106.160, 110.070 |
Unit cell angles | 61.17, 70.85, 86.21 |
Refinement procedure
Resolution | 19.990 - 1.800 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gx0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data scaling software | HKL-2000 |
Phasing software | X-PLOR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 236555 | |
Completeness [%] | 94.4 | 81.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |