2GWN
The structure of putative dihydroorotase from Porphyromonas gingivalis.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97874, 0.97912 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 86.459, 86.873, 114.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.100 - 1.850 |
| R-factor | 0.15099 |
| Rwork | 0.149 |
| R-free | 0.19372 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.379 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.100 | 1.940 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 36917 | |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 2M ammonium sulfate, 0.1M Cacodylate pH6.5, 0.1M NaCl, glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
