2GW8
Structure of the PII signal transduction protein of Neisseria meningitidis at 1.85 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.945 |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.141, 61.141, 48.074 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.480 - 1.850 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pil |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.065 | 0.418 |
| Number of reflections | 8803 | |
| <I/σ(I)> | 28.1 | 99.63 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.17M ammonium sulphate / 25.5% PEG4000 / 15% v/v glycerol, pH Unbuffered, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
