2GVZ

Crystal Structure of Complex of Gs- with The Catalytic Domains of Mammalian Adenylyl Cyclase: Complex with MANT-ATP and Mn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-24
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0781
Spacegroup name	P 21 21 2
Unit cell lengths	116.800, 132.100, 69.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.970 - 3.270
R-factor	0.275
Rwork	0.275
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tl7
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	3.270
Number of reflections	14536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	289	7.5-7.8 %PEG8000, 0.5M NACL, 0.1M PHOSPHATE BUFFER, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K