2GVN
L-asparaginase from Erwinia carotovora in complex with aspartic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.061, 135.880, 251.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.990 - 1.900 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zcf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.941 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.224 |
| Number of reflections | 191790 | |
| <I/σ(I)> | 31.3 | 5.5 |
| Completeness [%] | 97.2 | 88.1 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 12% MMEPEG 5000, 0.03% b-D-glucopiranoside, 0.02% sodium azide, 20 mm aspartic acid sodium salt, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
