2GVJ
Crystal Structure of Human NMPRTase in complex with FK866
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.780, 105.896, 83.427 |
| Unit cell angles | 90.00, 96.45, 90.00 |
Refinement procedure
| Resolution | 82.760 - 2.100 |
| R-factor | 0.25005 |
| Rwork | 0.247 |
| R-free | 0.29831 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.204 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.760 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 57137 | |
| Completeness [%] | 98.3 | 98.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.2 | 295 | 26% PEG 3350, 200 mM NaCL, BaCl2 as Additive, pH 9.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
